The influence of the hormonal changes that occur during menopause and its relation to Alzheimer’s Disease.
Embedded and mounted tissue, followed by slicing using the microtome, and relevant immunohistochemistry procedures.
Measured the level of specific hormones present in the plasma using ELISAs.
Trained in cell culture protocols.
Senior Honors Thesis: Using estrogen to rescue ABeta induced Alzheimer Disease related dysfunctional pathways.
Research Assistant
Summer
Mayo Clinic
PI: Wolfdieter Springer
Department of Neuroscience
Characterize the effect of higher PINK1 kinase activity of mitophagy and behavior during normal aging in a new mouse model both on the behavioral and biochemical level.
Processed and analyzed confocal microscopy images of different organs from a specific mouse model and quantified mitophagy.
Shadowed and assisted in the mice organ harvesting process, including the brain, heart, and muscle extraction.
Ran numerous Bicinchoninic Acid Protein Assays to determine protein concentration and proceed with tissue homogenization and Western Blot procedures.
Determined phosphorylated ubiquitin levels through running Meso Scale Discovery assays.
Research Assistant
Summer
National Institute of Medical Research
PI: Drenka Trivanovic
Department of Hematology and Stem Cells
Understanding the role of MSCs in aging, metabolic pathologies, and cancer.
Processed human white peripheral adipose tissue, and bone marrow adipose tissue to obtain MSCs.
Cultured cells, familiarizing myself with passing, seeding, treating and freezing procedures.
Processed confocal microscopy images using ImageJ MiNA toolset.
Research Assistant
to
University of Miami School of Medicine
PI: Michal Toborek
Department Biochemistry and Molecular Biology
The influence of HIV-1 on the function of brain endothelial extracellular vesicles and neural progenitor cells.
Trained in the use of confocal microscopy and image processing.
Carried out mitochondrial morphological analysis using imageJ.